DNA methylation profiling Gene specific profiling MG-63

Get tips on using E-Gel™ 50 bp DNA Ladder to perform DNA Ladder 50 bp

Get tips on using 100 bp DNA Ladder Ready to Load to perform DNA Ladder 100 bp

Get tips on using Quick-Load® 100 bp DNA Ladder to perform DNA Ladder 100 bp

Get tips on using Quick-Load® 100 bp DNA Ladder to perform DNA Ladder 100 bp

Get tips on using TriDye™ 1 kb Plus DNA Ladder to perform DNA Ladder 1 kb

Get tips on using Quick-Load® 1 kb DNA Ladder to perform DNA Ladder 1 kb

Get tips on using TrackIt™ 1 Kb Plus DNA Ladder to perform DNA Ladder 1 kb

Get tips on using GD 1Kb Plus DNA Ladder RTU Ladder to perform DNA Ladder 1 kb

Get tips on using MethylFlash Methylated DNA 5-mC Quantification Kit to perform DNA quantification Human - BJ

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.