Immunohistochemistry Vimentin (3B4) Mouse Human

Get tips on using ON-TARGETplus Human NCR3LG1 (374383) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HT-29 B7-H6/NCR3LG1

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse to perform Western blotting ERK

Get tips on using PerCP-Cy5-labeled CD66b to perform FACS CD66b - Mouse Human PerCP-Cy5

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using ON-TARGETplus Human IFIT1 (3434) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HFF1 IFIT1

Get tips on using ON-TARGETplus Human IFIT2 (3433) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HFF1 IFIT2

Get tips on using Human ShhN ELISA to perform ELISA Human - ShhN

Get tips on using Human ANGPTL3 ELISA to perform ELISA Human - Angiopoietin-Like 3 (AngptL3)