Site Directed Mutagenesis (SDM) Mouse 3T3-L1

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Get tips on using Monoclonal Mouse Anti-Human Cytokeratin 20 (Concentrate) Clone Ks20.8 to perform Immunohistochemistry Human - CK20

Products Agilent Technologies Monoclonal Mouse Anti-Human Cytokeratin 20 (Concentrate) Clone Ks20.8

Get tips on using Purified Mouse Anti-IKKγ Clone 54/IKKγ/NEMO (RUO) to perform Western blotting IKKgamma

Products BD Biosciences Purified Mouse Anti-IKKγ Clone 54/IKKγ/NEMO (RUO)

Get tips on using Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antibody to perform Western blotting AKT

Products R&D Systems Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antibody

Get tips on using Human/Mouse/Rat Total HSP70/HSPA1A DuoSet IC ELISA to perform ELISA Rat - HSP70

Products R&D Systems Human/Mouse/Rat Total HSP70/HSPA1A DuoSet IC ELISA

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

RNA siRNA / RNAi /miRNA transfection Mouse Primary Splenocytes Polymer / lipid

Get tips on using Pacific Blue™ anti-mouse Ly-6A/E (Sca-1) Antibody to perform Flow cytometry Anti-bodies Mouse - Ly-6A-E/Sca1

Products BioLegend Pacific Blue™ anti-mouse Ly-6A/E (Sca-1) Antibody

Get tips on using NAPSIN A (TMU-AD02) ANTI-HUMAN MOUSE IGG MOAB to perform Immunohistochemistry Human - Naspsin A

Products IBL, Immuno-Biological Laboratories co,Ltd NAPSIN A (TMU-AD02) ANTI-HUMAN MOUSE IGG MOAB

Get tips on using Monoclonal ANTI-FLAG® M2 antibody produced in mouse to perform ChIP Anti-bodies FLAG

Products Sigma-Aldrich Monoclonal ANTI-FLAG® M2 antibody produced in mouse

Get tips on using Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit to perform ELISA Rat - IGF-I

Products R&D Systems Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit

Microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

DNA Microarray RNA amplification & Labeling Mouse brain tissue Biotin

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