Site Directed Mutagenesis (SDM) Rat

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

Get tips on using HIF-1α siRNA (r) to perform siRNA / miRNA gene silencing Rat - Brain endothelial cells HIF-1α Lipid

Get tips on using C/EBP β siRNA (r) to perform siRNA / miRNA gene silencing Rat - Glial cells C/EBP‐β

Get tips on using FuGENE® 6 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat aortic smooth muscle cells (rASMC)

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rat pulmonary artery smooth muscle cells

Get tips on using Cell Death Detection ELISA to perform TUNEL assay cell type - Rat pulmonary arterial smooth muscle cells

Get tips on using FuGENE® 6 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat hepatic stellate cells

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat hepatic stellate cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rat brain microvascular endothelial cells

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary rat lymph node cells