Site Directed Mutagenesis (SDM) Rat Point mutation

Get tips on using BMP-2 Quantikine ELISA Kit to perform ELISA Rat - BMP-2

Get tips on using CD38 CRISPR Activation Plasmid (r) to perform CRISPR Rat - Activation CD38

Get tips on using Recombinant Anti-SOX9 antibody [EPR14335] (ab185230) to perform Immunohistochemistry Rat - Sox9

Get tips on using Recombinant Anti-PRMT5 antibody [EPR5772] (ab109451) to perform Immunohistochemistry Rat - PRMT5

Get tips on using 17 beta Estradiol ELISA Kit (ab108667) to perform ELISA Rat - Estradiol

Get tips on using Silencer® Select_Vamp2 siRNA(r) to perform siRNA / miRNA gene silencing Rat - RBL-2H3 Vamp2

Get tips on using Silencer® Select_Vamp7 siRNA(r) to perform siRNA / miRNA gene silencing Rat - RBL-2H3 Vamp7

Get tips on using Gibco™DMEM, low glucose, pyruvate to perform Stem cell culture media Rat TSPCs

Get tips on using HyClone™ Dulbecco's Modified Eagles Medium to perform Stem cell culture media Rat BMSC

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.