siRNA / miRNA gene silencing Rat H19-7

Get tips on using PAXgene Tissue RNA/miRNA Kit to perform RNA isolation / purification Tissue - Rat Lung

Get tips on using PAXgene Tissue RNA/miRNA Kit to perform RNA isolation / purification Tissue - Rat Liver

Get tips on using PAXgene Tissue RNA/miRNA Kit to perform RNA isolation / purification Tissue - Rat Kidney

Get tips on using PAXgene Tissue RNA/miRNA Kit to perform RNA isolation / purification Tissue - Rat Intestine

Get tips on using PAXgene Tissue RNA/miRNA Kit to perform RNA isolation / purification Tissue - Rat Heart

Get tips on using PAXgene Tissue RNA/miRNA Kit to perform RNA isolation / purification Tissue - Rat Brain

Get tips on using Glut1 siRNA and shRNA Plasmids (h) to perform siRNA / RNAi /miRNA transfection Human Cells - HT-1376 GLUT1

Get tips on using CD74 siRNA and shRNA Plasmids (h) to perform siRNA / RNAi /miRNA transfection Human Cells - HT-1376 CD74

Get tips on using PAXgene Tissue miRNA Kit to perform RNA isolation / purification Tissue - rat heart muscle tissue

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.