Wound healing assay cell type rat

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Get tips on using Anti-ATG4B antibody produced in rabbit to perform Autophagy assay cell type - MCF-7

Products Sigma-Aldrich Anti-ATG4B antibody produced in rabbit

Get tips on using LC3B (D11) XP® Rabbit mAb (Biotinylated) to perform Autophagy assay cell type - GL261

Products Cell Signaling Technology LC3B (D11) XP® Rabbit mAb (Biotinylated)

Get tips on using LC3A/B (D3U4C) XP® Rabbit mAb to perform Autophagy assay cell type - GL15

Products Cell Signaling Technology LC3A/B (D3U4C) XP® Rabbit mAb

Get tips on using LC3A/B (D3U4C) XP® Rabbit mAb to perform Autophagy assay cell type - U937

Products Cell Signaling Technology LC3A/B (D3U4C) XP® Rabbit mAb

Get tips on using Anti-p62/SQSTM1 antibody produced in rabbit to perform Autophagy assay cell type - HeLa

Products Sigma-Aldrich Anti-p62/SQSTM1 antibody produced in rabbit

Get tips on using LC3A/B (D3U4C) XP® Rabbit mAb to perform Autophagy assay cell type - A549

Products Cell Signaling Technology LC3A/B (D3U4C) XP® Rabbit mAb

Get tips on using DeadEnd™ Colorimetric TUNEL System to perform TUNEL assay cell type - FaDu human squamous cell carcinoma

Products Promega DeadEnd™ Colorimetric TUNEL System

Get tips on using Human/Mouse/Rat Activin A Quantikine ELISA Kit to perform ELISA Rat - Activin

Products R&D Systems Human/Mouse/Rat Activin A Quantikine ELISA Kit

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - Proximal tubular cells (rPT)

Products Sigma-Aldrich Anti-LC3B antibody produced in rabbit

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary rat astrocytes

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