siRNA / miRNA gene silencing Human SKOV-3

Get tips on using CytoSelect™ 24-Well Cell Migration Assay, 3 µm to perform Cell migration / Invasion cell type - isolated human neutrophils

Get tips on using Rac1/2/3 Antibody #2465 to perform Western blotting Rac1

Get tips on using Active/Pro-Caspase 3 Monoclonal Antibody (31A1067) to perform Western blotting Caspase-3

Get tips on using Quick Amp Labeling Kit-one color to perform RNA amplification & labeling Mammalian - miRNA, Human Endometrial Stromal cells Cyanine 3-pCp

Get tips on using Recombinant Anti-Caspase-3 antibody [E87] (ab32351) to perform Western blotting Caspase-3

Get tips on using Attractene Transfection Reagent to perform shRNA gene silencing Human - HEK 293T CAPN5- (Calpains) cationic lipid based

Get tips on using pSilencer 2.1-U6 Hygro to perform shRNA gene silencing Human - Neuroblastoma cells (SH-SY5Y) Beclin 1

Get tips on using Mouse Gene Expression v2 4x44K Microarray Kit to perform Microarray Gene expression arrays - Mouse liver tissue Cyanine-3-CTP

A key signature for necrotic cells is the permeabilization of the plasma membrane. Necrosis can be quantified by several cellular and biochemical assays. When studied minutely, it reveals the difficulty in confirmation in secondary induction of necrosis in apoptotic cells. Apoptotic cells are being analyzed to shift to necrotic status owing to membrane permeability at later stages, and thus, discrimination of two cell death becomes critical. Therefore, it is crucial to use a necrosis detection kit or a defined procedure to analyze this unprogrammed form of death in response to immense chemical and physical insults.