ChIP H3K27me3 Bovine Rat

Get tips on using p21 Waf1/Cip1 (12D1) Rabbit mAb #2947 to perform Western blotting p21

Get tips on using pET-28a-chBCO2 to perform Protein Expression Prokaryotic cells - E. coli chicken BCO2

Get tips on using Anti-CCSP - Rabbit to perform Flow cytometry Anti-bodies Mouse - CCSP

Get tips on using CD34 Monoclonal Antibody (RAM34), eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD34

Get tips on using Recombinant Anti-CD204/MSR1 Antibody (FITC), Rabbit Monoclonal to perform Flow cytometry Anti-bodies Mouse - CD204

Get tips on using Ras (D2C1) Rabbit mAb #8955 to perform Western blotting Ras

Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - Rat_Liver

Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - Rat_Circumvallate papillae

Get tips on using RIPA Buffer (10X) to perform Protein isolation Mammalian cells - Rat_Renal tissue

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.