ChIP H3K27me3 Human Mouse

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using MACSprep™ Chimerism CD34 MicroBead Kit, human to perform Cell Isolation CD34+ cells

Get tips on using Anti-phospho-Histone H2A.X (Ser139) Antibody to perform Immunohistochemistry γH2AX - Mouse Human -NA-

Get tips on using MAGnify™ Chromatin Immunoprecipitation System to perform ChIP Rat - Spinal cord

Get tips on using Imprint® Chromatin Immunoprecipitation Kit to perform ChIP Rat - Brain microvessels

Get tips on using Human ShhN ELISA to perform ELISA Human - ShhN

Get tips on using Bcl-11B (D6F1) XP® Rabbit mAb #12120 to perform ChIP Anti-bodies CtIP/BCL11A

Get tips on using Vimentin (D21H3) XP® Rabbit mAb to perform Immunohistochemistry Vimentin - Rabbit Human / mouse -NA-

Get tips on using Ki-67 Antigen, Clone MIB-1 to perform Immunohistochemistry Ki67 - Rabbit Mouse / Human -NA-

Get tips on using Human ANGPTL3 ELISA to perform ELISA Human - Angiopoietin-Like 3 (AngptL3)