Immunohistochemistry Collagen VII antibody [LH7.2] Mouse Human

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - HeLa

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - HeLa

Get tips on using FlowCellect Autophagy LC3 antibody based kit to perform Autophagy assay cell type - HeLa

Get tips on using Anti-LC3 antibody produced in rabbit to perform Autophagy assay cell type - L929

Get tips on using Anti-LC3 antibody produced in rabbit to perform Autophagy assay cell type - B16F10

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - H4

Get tips on using Anti-ATG4B antibody produced in rabbit to perform Autophagy assay cell type - H4

Get tips on using Anti-trimethyl-Histone H3 (Lys9) Antibody to perform ChIP H3K9me3 - Sheep Rat -NA-

Get tips on using Anti-trimethyl-Histone H3 (Lys4) Antibody to perform ChIP H3K4Me3 - Sheep Rat -NA-

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).