shRNA gene silencing Mouse Prostate cancer cell lines (DU145 and PC3)

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Protein isolation Tissue - Mouse skeletal muscle Experiment

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Proteins Protein isolation Tissue Mouse skeletal muscle

YAP siRNA (h) Product

Get tips on using YAP siRNA (h) to perform siRNA / miRNA gene silencing Human - OV2008 Yap Gene

Products Santa Cruz Biotechnology YAP siRNA (h)

CRISPR Mouse - Deletion RMA cells Trh4 Experiment

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

DNA CRISPR Mouse Deletion RMA cells Trh4

DeadEnd™ Fluorometric TUNEL System Product

Get tips on using DeadEnd™ Fluorometric TUNEL System to perform TUNEL assay cell type - HeLa cells human cervical cancer

Products Promega DeadEnd™ Fluorometric TUNEL System

Gibco™Advanced DMEM/F-12 Product

Get tips on using Gibco™Advanced DMEM/F-12 to perform 3D Cell Culture Media Human gastric cancer organoids