Get tips on using NucleoSpin® miRNA to perform RNA isolation / purification Cells - primary human mesenchymal stem cells
Protein ladders are a set of standards known as molecular weight proteins that are utilized to identify the approximate size of a protein molecule run on a PAGE gel electrophoresis. The challenges in running the ladders are the choice of appropriate protein standard as it is used as visual evidence of protein migration, transfer efficiency, and positive control. Suitable protein markers can be selected on the basis of required properties and applications, i.e., fluorescent ladder, IEF, 2D SDS-PAGE ladder, natural ladder with an isoelectric point, and optimized ladders for Western Blot chemiluminescence detection. The key factors for running a distinct protein ladder are buffer conditions, charge/voltage at migration time, and the gel's concentration.
Get tips on using pwPICZalpha-DT390-bi-pIL-2-Gly to perform Protein Expression Eukaryotic cells - P. pastoris Porcine IL-2 fusion toxins
Get tips on using Rac1/2/3 Antibody #2465 to perform Western blotting Rac1
Get tips on using ErbB2 (HER-2) Antibody Cocktail to perform Western blotting HER2
Get tips on using EpiTect 96 Bisulfite Kit (2) to perform Bisulfite DNA Modification Fluids
Get tips on using FaqI (BsmFI) (2 U/µL) to perform Restriction Enzymes BsmFI / FaqI
Get tips on using BspPI (AlwI) (2 U/µL) to perform Restriction Enzymes AlwI / BspPI
Get tips on using TIMP-2 Antibody (B-12): sc-365671 to perform Western blotting TIMP-2
Get tips on using Mouse BMP-2 PicoKine™ ELISA Kit to perform ELISA Mouse - BMP-2
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