Get tips on using PAXgene Tissue miRNA Kit to perform RNA isolation / purification Tissue - rat brain tissue
Get tips on using PAXgene Tissue miRNA Kit to perform RNA isolation / purification Tissue - rat spleen tissue
Get tips on using miRcute miRNA Isolation Kit to perform RNA isolation / purification Cells - immortalized MDA-MB-157
Get tips on using PAXgene Tissue miRNA Kit to perform RNA isolation / purification Tissue - rat heart muscle tissue
Get tips on using pSUPER.retro.puro to perform shRNA gene silencing Rat - MM1 SSH1
Get tips on using pSUPER.retro.puro to perform shRNA gene silencing Rat - MM1 SSH2
Get tips on using pSUPER.retro.puro to perform shRNA gene silencing Rat - MM1 LIMK1
Get tips on using pSUPER.retro.puro to perform shRNA gene silencing Rat - MM1 ADF
DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.
Get tips on using Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) to perform Flow cytometry Anti-bodies Mouse - CD16/CD32
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