CRISPR Mouse Deletion C2C12

Get tips on using pLV hUbC-dCas9-T2A-GFP to perform CRISPR Human - Repression HS2

Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Human - Repression miR130a

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Human - Repression EZH2

Get tips on using pHR-SFFV-dCas9-BFP-KRAB to perform CRISPR Human - Repression CXCR4

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Human - Repression HBV

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Get tips on using Phusion Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Human - Deletion MCF-7 sodium channel β1 subunit