The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
Get tips on using ViralSEQ™ Mouse Minute Virus (MMV) Detection System to perform Cell Culture Contamination Detection Kit Virus
Get tips on using PE Mouse Anti-Human CD61 Clone VI-PL2 to perform Flow cytometry Anti-bodies Human - CD61
Get tips on using PE Mouse Anti-Human CD26 Clone M-A261 to perform Flow cytometry Anti-bodies Human - CD26
Get tips on using PE-Cy™7 Mouse Anti-Human CD10 to perform Flow cytometry Anti-bodies Human - CD10
Get tips on using PE-Cy™7 Mouse Anti-Human CD56 to perform Flow cytometry Anti-bodies Human - CD56
Get tips on using PE-Cy™7 Mouse Anti-Human CD117 to perform Flow cytometry Anti-bodies Human - CD117
Get tips on using PE-Cy™7 Mouse Anti-Human CD28 to perform Flow cytometry Anti-bodies Human - CD28
Get tips on using PE Mouse Anti-Human CD30 Clone Ber-H83 to perform Flow cytometry Anti-bodies Human - CD30
Get tips on using PE-Cy™7 Mouse Anti-Human CD41a to perform Flow cytometry Anti-bodies Human - CD41
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