siRNA / RNAi /miRNA transfection Human Cells HT-1376

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - HT-29

Get tips on using BD Cycletest™ Plus DNA Kit to perform Cell cycle assay human - HT-29

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using Silencer® Select_Mapk14/p38 siRNA(r) to perform siRNA / miRNA gene silencing Rat - NRVM( Mapk14/p38

Get tips on using siGENOME Rat Arhgap35 (306400) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 p190RhoGAP/Arhgap35

Get tips on using siGENOME Rat Sod2 (24787) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - NRK MnSOD/Sod2

Get tips on using siGENOME Mouse Sod2 (20656) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - RGC-5 Sod2

Get tips on using ON-TARGETplus Rat Dlc1 (58834) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 Dlc1

Get tips on using ON-TARGETplus Rat Rhoa (117273) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 Rhoa

Get tips on using ON-TARGETplus Rat Rhoc (295342) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 RhoC