Protein Expression Prokaryotic cells L. citreum

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Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay luciferase human embryonic stem cells

Get tips on using Cell Lysis Buffer (10X) to perform Protein isolation Mammalian cells - THP-1

Products Cell Signaling Technology Cell Lysis Buffer (10X)

Get tips on using Cell Lysis Buffer (10X) to perform Protein isolation Mammalian cells - KC02-44D

Products Cell Signaling Technology Cell Lysis Buffer (10X)

Get tips on using NP40 Cell Lysis Buffer to perform Protein isolation Mammalian cells - BHK-21

Products Thermo Fisher Scientific NP40 Cell Lysis Buffer

Get tips on using RIPA Lysis and Extraction Buffer to perform Protein isolation Mammalian cells - HepG2

Products Thermo Fisher Scientific RIPA Lysis and Extraction Buffer

Get tips on using RIPA Lysis and Extraction Buffer to perform Protein isolation Mammalian cells - HEK293T

Products Thermo Fisher Scientific RIPA Lysis and Extraction Buffer

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Mammalian cells - HUVEC

Products Sigma-Aldrich CelLytic™ MT Cell Lysis Reagent

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Mammalian cells - HeLa

Products Sigma-Aldrich CelLytic™ MT Cell Lysis Reagent

Get tips on using QuantiPro™ BCA Assay Kit to perform Protein quantification Mammalian cells - L929

Products Sigma-Aldrich QuantiPro™ BCA Assay Kit

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay luciferase primary human endometrial stromal cells

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