Protein expression and purification Insect cells Hi5

Get tips on using TAGZyme pQE Vector Set to perform Protein tag Expression of His-tagged proteins

Get tips on using AllPrep DNA/RNA/Protein Mini Kit (50) to perform DNA isolation / purification Tissue - brain

Get tips on using Pre-designed and validated siRNA against gene IGFBP1 to perform siRNA / miRNA gene silencing Human - Primary Endometrial Stromal Cells IGFBP1 (Insuline-like growth factor binding protein-1)

Get tips on using pcDNA™3.1 (+) Mammalian Expression Vector to perform siRNA / miRNA gene silencing Human - U251 cofilin-1 (CFL1)

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - PC-12

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - Rat microglia

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - Human chondrocytes

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - BV-2

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - BV-2