siRNA / miRNA gene silencing Human Caki-2

DNA damage assay is a standard method for determining in-vivo/in-vitro genotoxicity by measuring the breaks in the DNA chain of animal and plant cells. Initial DNA damage leads to cell cycle arrest and, at the final stages, leads to induction of senescence or cell death (apoptosis, necrosis, autophagy, or mitotic catastrophe). Detection of DNA damage from mild to moderate to severe is challenging when studying genotoxicity in the pool of cells. It is favorable to use DNA damage assay kits available for prominent identification of the extent of damage in the analysis.

Get tips on using Stat3 Antibody (F-2): sc-8019 to perform Western blotting STAT3

Get tips on using E-cadherin monoclonal antibody (ECCD-2) to perform Western blotting E-cadherin

Get tips on using Mouse Lipocalin-2/NGAL DuoSet ELISA to perform ELISA Mouse - NGAL/LCN2

Get tips on using Laminin beta-2/gamma-1 Monoclonal Antibody (A5) to perform Western blotting Laminin subunit Beta-2

Get tips on using Laminin β-2 Antibody (H-1): sc-133241 to perform Western blotting Laminin subunit Beta-2

Get tips on using Smooth Muscle Cell Growth Medium 2 to perform Mammalian cell culture media HCASMC

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.