Get tips on using Mouse Myeloperoxidase DuoSet ELISA to perform ELISA Mouse - MPO
Get tips on using Mouse Leptin ELISA Kit to perform ELISA Mouse - Leptin
Get tips on using Mouse Decorin ELISA Kit to perform ELISA Mouse - Decorin
Get tips on using Mouse Osteopontin/OPN Antibody to perform Immunohistochemistry Mouse - Spp1/OPN
Get tips on using TLR10 shRNA (h) Lentiviral Particles to perform shRNA gene silencing Human - THP-1 TLR10
Get tips on using TLR10 shRNA (h) Lentiviral Particles to perform shRNA gene silencing Human - THP-1 TLR10
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
Get tips on using VENTANA anti-MSH2 (G219-1129) Mouse Monoclonal Primary Antibody to perform Immunohistochemistry Human - MSH2
Get tips on using pSilencer™ 4.1-CMV neo to perform shRNA gene silencing Human - SiHa AEG-1
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