siRNA / miRNA gene silencing Rat IEC-6

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

Get tips on using Rat TNF alpha ELISA Kit (ab46070) to perform ELISA Rat - TNF-alpha

Get tips on using Rat TNF-alpha Quantikine ELISA Kit to perform ELISA Rat - TNF-alpha

Get tips on using Rat beta NGF ELISA Kit (ab193736) to perform ELISA Rat - beta-NGF

Get tips on using Rat IGF-1 ELISA Kit (ab213902) to perform ELISA Rat - IGF-I

Get tips on using Rat / Mouse FGF-21 ELISA Kit to perform ELISA Rat - FGF-21

Get tips on using Rat Cytochrome c(CYCS) ELISA kit to perform ELISA Rat - Cytochrome c

Get tips on using Rat Bone Morphogenetic Protein 2 ELISA to perform ELISA Rat - BMP-2

Get tips on using Anti-LC3 (Rat) pAb to perform Autophagy assay cell type - OE21

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.