ChIP acH3 Mouse Canine

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Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Mouse - Deletion RAW 264.7 Dcstamp

Products Addgene pX330-U6-Chimeric_BB-CBh-hSpCas9

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Mouse - Deletion RAW 264.7 Nrp2

Products Addgene pX330-U6-Chimeric_BB-CBh-hSpCas9

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Mouse - Deletion 3T3-L1 MmP13

Products Addgene pX330-U6-Chimeric_BB-CBh-hSpCas9

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Mouse - Deletion ES (embryonic stem) cells Slx2

Products Addgene pX330-U6-Chimeric_BB-CBh-hSpCas9

I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

Discussions siRNA/RNAi/miRNA transfection human

Get tips on using PE/Cyanine7 anti-human CD14 Antibody to perform Flow cytometry Anti-bodies Human - CD14

Products BioLegend PE/Cyanine7 anti-human CD14 Antibody

Get tips on using PerCP/Cyanine5.5 anti-human CD49d Antibody to perform Flow cytometry Anti-bodies Human - CD49d

Products BioLegend PerCP/Cyanine5.5 anti-human CD49d Antibody

Get tips on using PerCP/Cyanine5.5 anti-human CD4 Antibody to perform Flow cytometry Anti-bodies Human - CD4

Products BioLegend PerCP/Cyanine5.5 anti-human CD4 Antibody

Get tips on using PerCP/Cyanine5.5 anti-human CD127 (IL-7Rα) Antibody to perform Flow cytometry Anti-bodies Human - CD127

Products BioLegend PerCP/Cyanine5.5 anti-human CD127 (IL-7Rα) Antibody

Get tips on using CD206 (MMR) Monoclonal Antibody (19.2), PE-Cyanine7, eBioscience™ to perform Flow cytometry Anti-bodies Human - CD206

Products eBioscience CD206 (MMR) Monoclonal Antibody (19.2), PE-Cyanine7, eBioscience™

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