Get tips on using CD206 Antibody, anti-human, PerCP-Vio® 700 to perform Flow cytometry Anti-bodies Human - CD206
Get tips on using Brilliant Violet 570™ anti-human CD27 Antibody to perform Flow cytometry Anti-bodies Human - CD27
Get tips on using Brilliant Violet 605™ anti-human CD69 Antibody to perform Flow cytometry Anti-bodies Human - CD69
Get tips on using PE/Dazzle™ 594 anti-human CD69 Antibody to perform Flow cytometry Anti-bodies Human - CD69
Get tips on using PerCP/Cyanine5.5 anti-human CD127 (IL-7Rα) Antibody to perform Flow cytometry Anti-bodies Human - CD127
Get tips on using Anti-Human L1CAM Therapeutic Antibody Fab Fragment to perform Flow cytometry Anti-bodies Human - CD171/L1CAM
Get tips on using Human IL-3R alpha /CD123 PE-conjugated Antibody to perform Flow cytometry Anti-bodies Human - CD123/IL3-R
Get tips on using Anti-phospho-Histone H2A.X (Ser139) Antibody to perform TissueFAxs phospho-Histone H2A.X (Ser139) - Mouse Human
Get tips on using Anti-phospho-Histone H2A.X (Ser139) Antibody to perform Immunofluorscence phospho-Histone H2A.X (Ser139) - Mouse Human
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
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