dna-isolation-purification-bacteria-gram-positive-clostridium-difficile

Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Tissue - human brain tissue

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Cells - primary human melanocytes

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - human liver tissue

Get tips on using HotStarTaq DNA Polymerase (25000) to perform PCR Hot start PCR - Bacterial DNA

Get tips on using HotStarTaq Plus DNA Polymerase to perform PCR Hot start PCR - Bacterial DNA

Get tips on using GoTaq® DNA Polymerase to perform PCR Conventional / Qualitative PCR - bacterial DNA

Get tips on using HotStarTaq Plus DNA Polymerase to perform PCR Conventional / Qualitative PCR - bacterial DNA

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. A quantitative, real-time PCR reaction typically includes all of that plus a probe that can be detected fluorescently as the reaction runs, with no gel required. for detection. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Cells - primary human pulmonary artery endothelial cells

Get tips on using EZ-10 Spin Column PCR Product Purification kit to perform DNA gel extraction / PCR product purification Product size > 15Kb