siRNA / RNAi /miRNA transfection Rat IEC-6

Get tips on using Rat CX3CL1/Fractalkine DuoSet ELISA to perform ELISA Rat - CX3CL1

Get tips on using Rat Adiponectin ELISA Kit (ab108784) to perform ELISA Rat - Adiponectin

Get tips on using Viromer® RED to perform DNA transfection Mammalian cells - Primary cells Rat astrocytes

Get tips on using Rat Transferrin ELISA Kit (ab137993) to perform ELISA Rat - Transferrin (Tf)

Get tips on using Rat Transferrin ELISA Kit (Colorimetric) to perform ELISA Rat - Transferrin (Tf)

Get tips on using Rat beta-NGF DuoSet ELISA to perform ELISA Rat - beta-NGF

Get tips on using HO-1 (rat), ELISA kit to perform ELISA Rat - HO-1

Get tips on using Silencer® Select GLO-1 siRNA to perform siRNA / miRNA gene silencing Human - Primary Human Aortic Endothelial Cells GLO-1 Lipid

Get tips on using Rat ICAM1 ELISA Kit (CD54) (ab100763) to perform ELISA Rat - ICAM-1/CD54

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.