siRNA / miRNA gene silencing Human BC-1

Get tips on using CD74 siRNA and shRNA Plasmids (h) to perform RNA sequencing Human - HT-1376 (urinary bladder cell line)

Get tips on using EMBacY#1 to perform Protein Expression Eukaryotic cells - Hi5 mDicer2

Get tips on using Torin 1 to perform Autophagy assay cell type - Mel cells

Get tips on using 1 Kb DNA Ladders to perform DNA Ladder 1 kb

Get tips on using 1 kb DNA Ladder to perform DNA Ladder 1 kb

Get tips on using Claudin 1 Polyclonal Antibody to perform Western blotting Cclaudin-1

Get tips on using HIF-1 alpha Antibody to perform Western blotting HIF-1 alpha

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Get tips on using Rock-2 siRNA and shRNA Plasmids (h) to perform RNA sequencing Human - HT-1376 (urinary bladder cell line)

Get tips on using pRSET A-FhFtn-1 to perform Protein Expression Prokaryotic cells - E. coli FhFtn-1