siRNA / miRNA gene silencing Human KLM-1

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Notch 1 Antibody (A-8): sc-376403 to perform Immunohistochemistry Human - Notch1

Get tips on using miRNA Complete Labeling and Hyb Kit to perform Microarray RNA amplification & Labeling - Human endometrial stromal cells Cyanine 3-pCp

Get tips on using Beclin-1 Antibody to perform Autophagy assay cell type - THP 1

Get tips on using Glut1 siRNA and shRNA Plasmids (h) to perform RNA sequencing Human - HT-1376 (urinary bladder cell line)

Get tips on using CD74 siRNA and shRNA Plasmids (h) to perform RNA sequencing Human - HT-1376 (urinary bladder cell line)

Get tips on using EMBacY#1 to perform Protein Expression Eukaryotic cells - Hi5 mDicer2

Get tips on using Torin 1 to perform Autophagy assay cell type - Mel cells

Get tips on using 1 Kb DNA Ladders to perform DNA Ladder 1 kb

Get tips on using 1 kb DNA Ladder to perform DNA Ladder 1 kb