siRNA / miRNA gene silencing Rat INS-1

Get tips on using Mouse Rat Estradiol ELISA Kit to perform ELISA Rat - Estradiol

Get tips on using Rat CX3CL1/Fractalkine DuoSet ELISA to perform ELISA Rat - CX3CL1

Get tips on using Rat Adiponectin ELISA Kit (ab108784) to perform ELISA Rat - Adiponectin

Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Cells - immortalized PK-1

Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Cells - immortalized THP-1

Get tips on using Rat Transferrin ELISA Kit (ab137993) to perform ELISA Rat - Transferrin (Tf)

Get tips on using Rat Transferrin ELISA Kit (Colorimetric) to perform ELISA Rat - Transferrin (Tf)

Get tips on using Rat beta-NGF DuoSet ELISA to perform ELISA Rat - beta-NGF

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.