CRISPR Mouse Deletion B16-F1

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Get tips on using hCas9 to perform CRISPR Mouse - Deletion B117P Dck

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using hCas9 to perform CRISPR Mouse - Deletion Dck

Get tips on using JAM-A CRISPR/Cas9 KO Plasmid (h) to perform CRISPR Human - Deletion F11R

Get tips on using hCas9 to perform CRISPR Mouse - Deletion C2C12 Dmd

Get tips on using pCas9_GFP to perform CRISPR Mouse - Deletion ANS4 Rosa26

Get tips on using JDS246 to perform CRISPR Mouse - Deletion C2C12 Prnp

Get tips on using MLM3636 to perform CRISPR Mouse - Deletion Neuro 2a Prnp

Get tips on using JDS246 to perform CRISPR Mouse - Deletion Neuro 2a Prnp