cell-cycle-assay-human-hela

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Wound healing assay can be challenging due to inconsistencies and variations while making a wound on the confluent cell monolayer, consequently leads to wounds of varying sizes and widths. Moreover, this assay causes damage to the cells that are at the edge of the wound, which can prevent cell migration into the wound site and healing. The best solution is to use the standard wound healing assay kits using either combs or inserts to make a defined wound field or gap and prevent the well-to-well variation in these assays.

Cellular assays Wound healing assay cell type human MDA-MB-231
Passage number Discussion

HeLa cells I used are passage 40. Are these cells good to perform Cell cycle analysis on?

Discussions Passage number

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Cellular assays TUNEL assay cell type FaDu human squamous cell carcinoma

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay HeLa

Products Bio-Techne CometAssay Single Cell Gel Electrophoresis Assay

Get tips on using ORIS™ Cell Migration Assay to perform Cell migration / Invasion cell type - HeLa

Products Platypus Technologies ORIS™ Cell Migration Assay

Get tips on using Click-iT™ EdU Pacific Blue™ Flow Cytometry Assay Kit to perform Cell cycle assay human - MCF 10A

Products Thermo Fisher Scientific Click-iT™ EdU Pacific Blue™ Flow Cytometry Assay Kit

Get tips on using Click-iT™ EdU Pacific Blue™ Flow Cytometry Assay Kit to perform Cell cycle assay human - PANC-1

Products Thermo Fisher Scientific Click-iT™ EdU Pacific Blue™ Flow Cytometry Assay Kit

Get tips on using Click-iT™ Plus EdU Pacific Blue™ Flow Cytometry Assay Kit to perform Cell cycle assay human - A549

Products Thermo Fisher Scientific Click-iT™ Plus EdU Pacific Blue™ Flow Cytometry Assay Kit

Get tips on using Click-iT™ Plus EdU Pacific Blue™ Flow Cytometry Assay Kit to perform Cell cycle assay human - SKOV3

Products Thermo Fisher Scientific Click-iT™ Plus EdU Pacific Blue™ Flow Cytometry Assay Kit

Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - HeLa cells human cervical cancer

Products Sigma-Aldrich In Situ Cell Death Detection Kit, Fluorescein

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