Site Directed Mutagenesis (SDM) Rat Deletion H9C2

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Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Rat - Mesenteric arteries

Products Active Motif ChIP-IT® Express Chromatin Immunoprecipitation Kits

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Rat - INS-1

Products Active Motif ChIP-IT® Express Chromatin Immunoprecipitation Kits

Get tips on using Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 to perform Immunohistochemistry Rat - GFAP

Products Merck Millipore Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5

Get tips on using EZ-Magna ChIP™ G - Chromatin Immunoprecipitation Kit to perform ChIP Rat - Liver

Products Merck Millipore EZ-Magna ChIP™ G - Chromatin Immunoprecipitation Kit

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

DNA DNA gel extraction / PCR product purification Product size < 15Kb

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

DNA DNA gel extraction / PCR product purification Product size > 15Kb

Get tips on using HIF-1α siRNA (r) to perform siRNA / miRNA gene silencing Rat - Brain endothelial cells HIF-1α Lipid

Products Santa Cruz Biotechnology HIF-1α siRNA (r)

Get tips on using C/EBP β siRNA (r) to perform siRNA / miRNA gene silencing Rat - Glial cells C/EBP‐β

Products Santa Cruz Biotechnology C/EBP β siRNA (r)

Get tips on using FuGENE® 6 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat aortic smooth muscle cells (rASMC)

Products Promega FuGENE® 6 Transfection Reagent

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rat pulmonary artery smooth muscle cells

Products Qiagen RNeasy Mini Kit

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