siRNA / RNAi /miRNA transfection Human Cells KG-1

Get tips on using Lipofectamine® LTX Reagent to perform DNA transfection Mammalian cells - Primary cells Human aortic smooth muscle cells (HOSMC)

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using Silencer® Select_Vamp2 siRNA(r) to perform siRNA / miRNA gene silencing Rat - RBL-2H3 Vamp2

Get tips on using Silencer® Select_Vamp7 siRNA(r) to perform siRNA / miRNA gene silencing Rat - RBL-2H3 Vamp7

Get tips on using IGF-IRα/β siRNA (r) to perform siRNA / miRNA gene silencing Rat - RGC-5 IGF1R

Get tips on using ON-TARGETplus Mouse Cflar (12633) siRNA to perform siRNA / miRNA gene silencing Mouse - 3T3-SA Flip/CFLAR

Get tips on using GATA-1 shRNA Plasmids (h) to perform shRNA gene silencing Human - TF‐1 GATA‐1

Get tips on using ON-TARGETplus Rat Becn1 (114558) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - UMR‐106 Becn1

Get tips on using siGENOME Rat Lrp5 (293649) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - NPC Lrp5

Get tips on using siGENOME Rat Lrp6 (312781) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - NPC Lrp6