DNA gel extraction / PCR product purification

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Get tips on using X-tremeGENE™ HP DNA Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines H9C2

Products Sigma-Aldrich X-tremeGENE™ HP DNA Transfection Reagent

Get tips on using X-tremeGENE™ HP DNA Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines Huh7

Products Sigma-Aldrich X-tremeGENE™ HP DNA Transfection Reagent

Get tips on using X-tremeGENE™ HP DNA Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines HepG2

Products Sigma-Aldrich X-tremeGENE™ HP DNA Transfection Reagent

Get tips on using X-tremeGENE™ HP DNA Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines PANC-1

Products Sigma-Aldrich X-tremeGENE™ HP DNA Transfection Reagent

Get tips on using X-tremeGENE™ HP DNA Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines MDA-MB-231

Products Sigma-Aldrich X-tremeGENE™ HP DNA Transfection Reagent

Get tips on using X-tremeGENE™ HP DNA Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Human aortic smooth muscle cells (HOSMC)

Products Sigma-Aldrich X-tremeGENE™ HP DNA Transfection Reagent

Get tips on using ApoBrdU Red DNA Fragmentation Kit to perform TUNEL assay cell type - SKOV3, Caov3 human ovarian cancer

Products Biovision ApoBrdU Red DNA Fragmentation Kit

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Cells - Primary cells Rat astrocytes

Products Qiagen DNeasy Blood & Tissue Kit

Get tips on using Quick-Load® Purple 1 kb DNA Ladder to perform DNA Ladder 1 kb

Products New England BioLabs Quick-Load® Purple 1 kb DNA Ladder

I am currently using a recombinant protein which shows metal-dependent DNase activity. Is it possible to pinpoint the source of the DNase activity after protein purification? More specifically, can I ensure that the DNase activity is not because of nuclease contamination from the E.coli that might have persisted and passed with the protein of interest during purification?

Discussions Is a bacterial nuclease contamination possible during protein purification?

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