rna-isolation-purification-cells-primary-rat-cortical-neurons

Get tips on using Gibco™DMEM/F-12 to perform Stem cell Differentiation media hESCs differentiation into cortical neuroepithelium (NE)

Get tips on using Gibco™Glasgow's MEM (GMEM) to perform Stem cell Differentiation media hESCs differentiation into cortical neuroepithelium (NE)

Get tips on using Gibco™KnockOut™ Serum Replacement to perform Stem cell Differentiation media hESCs differentiation into cortical neuroepithelium (NE)

Get tips on using BioMag Goat Anti-Rat IgG (500 ml) to perform Cell Isolation Mouse T cells

Get tips on using jetPEI® DNA transfection, HTS application to perform DNA transfection Mammalian cells - Primary cells Rat dermal fibroblasts (rDF)

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - Corticospinal motor neurons

Get tips on using ISOGEN to perform RNA isolation / purification Cells - immortalized CHO