Immunohistochemistry Anti-mouse IgG Donkey Mouse

Get tips on using Recombinant Anti-Integrin beta 1 antibody [EPR16895] (ab179471) to perform Western blotting ITGB1

Get tips on using Anti-Human L1CAM Therapeutic Antibody Fab Fragment to perform Flow cytometry Anti-bodies Human - CD171/L1CAM

Get tips on using PE anti-human CD111 (Nectin-1) Antibody to perform Flow cytometry Anti-bodies Human - CD111/Nectin-1

Get tips on using PE anti-human CD126 (IL-6Rα) Antibody to perform Flow cytometry Anti-bodies Human - CD126/IL-6Ralpha

Get tips on using Anti-Beclin 1 antibody (ab62557) to perform Autophagy assay cell type - Proximal tubular cells (rPT)

Get tips on using Anti-LC3 (Rat) pAb to perform Autophagy assay cell type - OE21

Get tips on using Anti-LC3 (Rat) pAb to perform Autophagy assay cell type - MLO-Y4

Get tips on using Anti-LC3 (Rat) pAb to perform Autophagy assay cell type - MLO-Y4

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.