Immunohistochemistry Anti-mouse IgG Donkey Mouse

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.

Get tips on using Recombinant Anti-PCNA antibody [EPR3821] (ab92552) to perform Western blotting PCNA

Get tips on using Anti-Cleaved PARP1 antibody [4B5BD2] (ab110315) to perform Western blotting PARP

Get tips on using Recombinant Anti-Bax antibody [E63] (ab32503) to perform Western blotting Bax

Get tips on using Recombinant Anti-SOX9 antibody [EPR14335] (ab185230) to perform Western blotting SOX9

Get tips on using Anti-Src (phospho Y418) antibody (ab4816) to perform Western blotting Src

Get tips on using Anti-SMAD1/5/9 antibody (ab66737) to perform Western blotting Smad1

Get tips on using Recombinant Anti-Smad4 antibody [EP618Y] (ab40759) to perform Western blotting Smad4

Get tips on using Anti-Smad2 (phospho S467) antibody (ab53100) to perform Western blotting Smad2