siRNA / miRNA gene silencing Mouse Embryonic stem cells

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DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

DNA Microarray Gene expression arrays Human whole blood cells Biotin

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized EBL (embryonic lung cell)

Products Qiagen RNeasy Mini Kit

Get tips on using CellGenix® GMP SCGM Stem Cell Growth Medium to perform Mammalian cell culture media NK-92

Products CellGenix CellGenix® GMP SCGM Stem Cell Growth Medium

Cellular assays Cell Isolation Breast Cancer Stem Cell

Cellular assays Cell Isolation Human Mesenchymal Stem Cell

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell culture media Mouse myoblasts cells

Products Fisher Scientific Gibco™ DMEM, high glucose

Get tips on using TransIT-TKO Transfection Reagent to perform siRNA / RNAi /miRNA transfection Mouse - Primary Splenocytes Polymer / lipid

Products Mirus TransIT-TKO Transfection Reagent

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Whole genome profiling - mouse hematopoietic stem cells

Products Zymo Research EZ DNA Methylation kit

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell culture media Mouse muscle satellite cells

Products Fisher Scientific Gibco™ DMEM, high glucose

Get tips on using Galacto-Star™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells to perform Reporter gene assay β-galactosidase substrates - BHK-21 baby hamster kidney cells

Products Thermo Fisher Scientific Galacto-Star™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells

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