Protein quantification Mammalian cells

Get tips on using pGS-CD62L to perform Protein Expression Eukaryotic cells - CHO CD62L

Get tips on using pMH3-rFVII to perform Protein Expression Eukaryotic cells - CHO rFVII

Get tips on using pLEXSY-hyg-2-hAm to perform Protein Expression Eukaryotic cells - Iranian lizard Leishmania cells recombinant human amelogenin

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using pGETS118 to perform Protein Expression Prokaryotic cells - B. subtilis cellulosomal complexes

Get tips on using pBGP3-RsEG to perform Protein Expression Eukaryotic cells - P. pastoris cellulases

Get tips on using pBGP3-NtEG to perform Protein Expression Eukaryotic cells - P. pastoris cellulases

Get tips on using pBGP3-G1NkBG to perform Protein Expression Eukaryotic cells - P. pastoris cellulases

Get tips on using pBGP3-G1mgNtBG1 to perform Protein Expression Eukaryotic cells - P. pastoris cellulases

Get tips on using pANC233 (xylG) to perform Protein Expression Prokaryotic cells - A. cellulolyticus xylG