RNA sequencing Mouse

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Mouse Heart

Get tips on using RNeasy Micro Kit to perform RNA isolation / purification Tissue - Mouse Eye

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Mouse Ear

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Mouse Colon

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Mouse Decidua

Get tips on using RNeasy Micro Kit to perform RNA isolation / purification Tissue - Mouse Cornea

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Mouse Brainstem

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Mouse Bone

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Mouse Brain

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).