CRISPR Mouse Deletion RMA cells

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Get tips on using Gibco™ DMEM, high glucose to perform Stem cell culture media Mouse myoblasts cells

Products Fisher Scientific Gibco™ DMEM, high glucose

DNA DNA isolation / purification Cells Primary cells Buccal cells

Get tips on using pET-21b(+)-mTNFα to perform Protein Expression Prokaryotic cells - E. coli mouse TNF-(α)

Products Mojtaba Sankian, Immuno-Biochemistry lab, Immunology Research Ce pET-21b(+)-mTNFα

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - Mouse macrophages

Products Thermo Fisher Scientific Pierce™ BCA Protein Assay Kit

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - Mouse microglia

Products Thermo Fisher Scientific Pierce™ BCA Protein Assay Kit

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

DNA DNA transfection Mammalian cells Primary cells Rat pulmonary artery smooth muscle cell (pPASMC)

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary bovine coronary artery smooth muscle cells

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary bovine pulmonary artery smooth muscle cells

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary canine coronary artery smooth muscle cells

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary human hair follicle dermal papilla cells

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