siRNA / miRNA gene silencing Human PC3 (human prostate cancer cell line)

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Human liver cancer organoids

Get tips on using Advanced DMEM to perform 3D Cell Culture Media Human cancer esophageal organoids

Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - A549, NCI-H460, H1299 human lung cancer cells

Get tips on using in situ Cell Death Detection Kit, POD to perform TUNEL assay cell type - A549, NCI-H460, H1299 human lung cancer cells

Get tips on using EasySep™ Direct Human Naïve B Cell Isolation Kit to perform Cell Isolation Naive B cell

Get tips on using 3D-Gene® Mouse miRNA Oligo chip (ver.21) to perform Microarray Gene expression arrays - Mouse liver tissue Cyanine-3-CTP

Get tips on using MACSxpress Whole Blood Pan T Cell Isolation Kit, human to perform Cell Isolation Naive Pan T cell

Get tips on using EasySep™ Human Naïve Pan T Cell Isolation Kit to perform Cell Isolation Naive Pan T cell

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.