siRNA / miRNA gene silencing Rat RMC-1

Get tips on using PE Rat anti-Mouse CD34 to perform Flow cytometry Anti-bodies Mouse - CD34

Get tips on using Biotin Rat Anti-Mouse CD45 to perform Flow cytometry Anti-bodies Mouse - CD45

Get tips on using FITC Rat Anti-Human CD49f to perform Flow cytometry Anti-bodies Human - CD49f/ITGA6

Get tips on using PE Rat Anti-Mouse CD184 to perform Flow cytometry Anti-bodies Mouse - CD184/CXCR4

Get tips on using PE Rat anti-Mouse CD146 to perform Flow cytometry Anti-bodies Mouse - CD146/MCAM

Get tips on using Purified Rat Anti-Mouse CD117 to perform Flow cytometry Anti-bodies Mouse - CD117/c-kit

Get tips on using PE Rat Anti-Mouse CD140A to perform Flow cytometry Anti-bodies Mouse - CD140/PDGFR-α

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using Rat Retinol binding protein 4,RBP-4 ELISA Kit to perform ELISA Rat - RBP4

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.