Protein expression and purification Insect cells S2

Get tips on using PageRuler™ Unstained Protein Ladder to perform Protein Ladder Unstained

Get tips on using IRIS9 Plus Prestained Protein Ladder to perform Protein Ladder Prestained

Get tips on using iBright™ Prestained Protein Ladder to perform Protein Ladder Prestained

Get tips on using CozyHi™ Prestained Protein Ladder to perform Protein Ladder Prestained

Get tips on using CozyXL™ Prestained Protein Ladder to perform Protein Ladder Prestained

Get tips on using Cozy™ Prestained Protein Ladder to perform Protein Ladder Prestained

Get tips on using EZQ™ Protein Quantitation Kit to perform Protein quantification Fluorimetric method

Get tips on using Qubit™ Protein Assay Kit to perform Protein quantification Fluorimetric method

Get tips on using NanoOrange™ Protein Quantitation Kit to perform Protein quantification Fluorimetric method

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.