shRNA gene silencing Mouse RGC-5

Get tips on using Accell Human VDAC1 (7416) siRNA - Set of 4 to perform siRNA / miRNA gene silencing Human - A549 VDAC1

Get tips on using NeuroCult™ Basal Medium (Mouse & Rat) to perform 3D Cell Culture Media Mouse embryonic neurospheres

Get tips on using Purified anti-mouse TNF-α Antibody to perform Flow cytometry Anti-bodies Mouse - TNF-α

Get tips on using FITC anti-mouse CD95 (Fas) Antibody to perform Flow cytometry Anti-bodies Mouse - CD95/Fas

Get tips on using APC anti-mouse CD252 (OX40L) Antibody to perform Flow cytometry Anti-bodies Mouse - CD252/OX40L

Get tips on using Purified Rat Anti-Mouse IFN-γ to perform Flow cytometry Anti-bodies Mouse - IFN-γ

Get tips on using APC Rat Anti-Mouse IFN-γ to perform Flow cytometry Anti-bodies Mouse - IFN-γ

Get tips on using Purified Rat Anti-Mouse Siglec-F to perform Flow cytometry Anti-bodies Mouse - Siglec F

Get tips on using BV421 Rat Anti-Mouse Siglec-F to perform Flow cytometry Anti-bodies Mouse - Siglec F

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).