siRNA / miRNA gene silencing Human MCF-7

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Get tips on using Human Genome CGH Microarray Kit, 4x44K to perform Microarray Gene expression arrays - A-375 human melanoma Digoxigenin-11-dUTP

Get tips on using IL-4Rα shRNA (m) Lentiviral Particles to perform shRNA gene silencing Mouse - R221a IL4Rα

Get tips on using INTERFERin® to perform siRNA / RNAi /miRNA transfection Mouse - B16 Polymer / lipid

Get tips on using AM5770: pSilencer™ 3.1-H1 neo to perform shRNA gene silencing Mouse - CT26 OPN

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - immortalized MCF 10A

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized MCF 10A

Get tips on using MEGM-Mammary-Epithelial-Cell-Growth-Medium to perform Mammalian cell culture media MCF-10A