Immunohistochemistry Wilms Tumor 1 (WT1) Rabbit Mouse

Get tips on using Glucocorticoid Receptor (D8H2) XP® Rabbit mAb #3660 to perform Immunohistochemistry Human - GR/glucocorticoid receptor

Get tips on using Anti-alpha smooth muscle Actin antibody to perform Immunohistochemistry Alpha smooth muscle Actin - Rabbit Mouse -NA-

Get tips on using CONFIRM anti-Estrogen Receptor (ER) (SP1) Rabbit Monoclonal Primary Antibody to perform Immunohistochemistry Human - ER

Get tips on using Mouse KIM-1 ELISA Kit to perform ELISA Mouse - KIM-1

Get tips on using Mouse Dkk-1 ELISA Kit to perform ELISA Mouse - Dkk-1

Get tips on using CD133 (Prominin-1) Monoclonal Antibody (13A4), APC, eBioscience™ to perform Immunohistochemistry Mouse - CD133

Get tips on using Mouse Dkk-1 Quantikine ELISA Kit to perform ELISA Mouse - Dkk-1

Get tips on using Anti-CCSP - Rabbit to perform Flow cytometry Anti-bodies Mouse - CCSP

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using Mouse KIM1 ELISA Kit (TIM-1) (ab119596) to perform ELISA Mouse - KIM-1