CRISPR Mouse Deletion RMA cells

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Mouse primary lung epithelial cells-organoids

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Mouse primary mammary ephitelial cells- organoids

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell culture media Mouse muscle satellite cells

Get tips on using Gibco™ RPMI 1640 Medium to perform Stem cell culture media Mouse trophoblast stem cells

Get tips on using Gibco™DMEM/F-12 to perform Stem cell culture media Mouse trophoblast stem cells

Get tips on using DeadEnd™ Colorimetric TUNEL System to perform TUNEL assay cell type - Mouse skeletal muscle cells

Get tips on using EpiTect Bisulfite Kit to perform DNA methylation profiling Whole genome profiling - mouse primordial germ cells

Get tips on using Stealth siRNA(m) Atg16l2 to perform siRNA / miRNA gene silencing Mouse - Pancreatic Acinar cells Atg16l2

Get tips on using SMARTpool: siGENOME Fancd2 siRNA to perform siRNA / miRNA gene silencing Mouse - B16 Melanoma cells FANCD2

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.