rna-isolation-purification-cells-primary-mouse-ventricles

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TRIzol Reagent Product

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized CMT12

Products Thermo Fisher Scientific TRIzol Reagent

DNA isolation / purification Bacteria - Gram negative Massilia sp Experiment

DNA DNA isolation / purification Bacteria Gram negative Massilia sp

DNA isolation / purification Bacteria - Gram negative Salmonella typhi Experiment

DNA DNA isolation / purification Bacteria Gram negative Salmonella typhi

DNA isolation / purification Bacteria - Gram positive Clostridium botulinum Experiment

DNA DNA isolation / purification Bacteria Gram positive Clostridium botulinum

ChIP Mouse - 3T3-L1 cells Experiment

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Proteins ChIP Mouse 3T3-L1 cells

RNeasy Mini Kit Product

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized NHA