siRNA / RNAi /miRNA transfection Rat

Get tips on using Rat Lipocalin-2/NGAL PicoKine™ ELISA Kit to perform ELISA Rat - NGAL/LCN2

Get tips on using Rat KIM-1 TIM-1 ELISA Kit (ab119597) to perform ELISA Rat - KIM-1

Get tips on using Rat heme oxygenase 1,HO-1 ELISA Kit to perform ELISA Rat - HO-1

Get tips on using Rat soluble E-selectin,sE-selectin ELISA Kit to perform ELISA Rat - sE-Selectin

Get tips on using Mouse/Rat Leptin Quantikine ELISA Kit to perform ELISA Mouse - Leptin

Get tips on using Active BDNF (Human, Rat) ELISA Kit to perform ELISA Mouse - GDNF

Get tips on using Rat TGF Beta 1 PicoKine™ ELISA Kit to perform ELISA Rat - TGF-beta 1

Get tips on using Rat Superoxide dismutase [Mn], mitochondrial(SOD2) ELISA kit to perform ELISA Rat - SOD2/Mn-SOD

Get tips on using Rat IL-1 Beta PicoKine™ ELISA Kit to perform ELISA Rat - IL-1 beta

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.